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pYHT1
pYHT1
規(guī)格:
貨期:
編號(hào):B244655
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pYHT1
商品貨號(hào) B244655
Designations pYHT1
GenBank Number

X58156

Species Homo sapiens, human
Vector
Construct size (kb): 9.899999618530273
Insert
DNA: genomic
Insert lengths(kb): 3.700000047683716
Tissue: fibroblast GM3468 cell line
Gene product: subtelomeric and telomeric repeats
Insert Size (kb) 3.700
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): BglII--7.2, 2.9; EcoRI--10.0, HindIII--5.2, 2.9, 1.8; XhoI--10.0; PstI--3.8, 2.4, 1.7, 1.3, 0.8.
The insert contains the following restriction sites (kb from the EcoRI end): PvuII--0.617, 1.838, 2.447; BglII--0.693, 0.719, 0.801; KpnI--1.324, 3.248; PstI--1.613, 1.833, 2.617, 3.497.
The KpnI/PstI fragment (probe A1, nt 3249-3497) and the PvuII/KpnI fragment (probe A2, nt 2448-3248) cross-hybridize to hamster DNA and detect a number of variable fragments in human DNA. A2 contains a CpG island.
The PvuII fragment (probe B, nt 1838-2448) does not cross-hybridize to hamster DNA.
The EcoRI/BglII fragment (probe C, nt 1-693) does not cross-hybridize to hamster DNA. It detects a 6.0 kb poly(A)+ RNA in HeLa cells.
The Alu repeat extends from nt 1368 to 1695.
Generated from yHT1 (a 5.5 kb EcoRI/telomeric fragment ligated to the EcoRI/BamHI fragment of pYAC4) by digesting the linear DNA with Bal31 and self-ligating.
There are residual yeast/Tetrahymena telomeric repeats located next to the vector HindIII site which is 0.9 kb from the PstI site at nt 3499 of the insert.
References

Cheng JF, et al. Structural and transcriptional analysis of a human subtelomeric repeat. Nucleic Acids Res. 19: 149-154, 1991. PubMed: 2011494

Cheng JF, et al. Isolation and characterization of a human telomere. Nucleic Acids Res. 17: 6109-6127, 1989. PubMed: 2549507

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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