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pHPT31
pHPT31
規(guī)格:
貨期:
編號:B236910
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 pHPT31
商品貨號 B236910
Designations pHPT31
Species Homo sapiens, human
Insert
DNA: cDNA
Insert lengths(kb): 0.949999988079071
Tissue: central nervous system
Gene product: hypoxanthine phosphoribosyltransferase [HPRT]
Alleles: A1, A2, A3
Insert Size (kb) 0.950
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--2.5, 1.0; SalI--2.25, 0.95; PstI--2.5, 0.93; EcoRI--2.4, 0.99.
The clone was constructed by excising the PstI fragment encoding the full-length cDNA from pHPT30 and inserting the fragment into pUC7. The insert includes about 100 bp 5' untranslated and some 3' untranslated sequence, but not the AATAAA sequence.
The insert detects the same BamHI polymorphism detected by the probe, pDSK1.
Enzyme(s) not detecting polymorphism: EcoRI, HindIII, MspI, KpnI, TaqI.
References

Jolly DJ, et al. Isolation and characterization of a full-length expressible cDNA for human hypoxanthine phosphoribosyltransferase. Proc. Natl. Acad. Sci. USA 80: 477-481, 1983. PubMed: 6300847

Brennand J, et al. Expression of human and Chinese hamster hypoxanthine-guanine phosphoribosyltransferase cDNA recombinants in cultured Lesch-Nyhan and Chinese hamster fibroblasts. J. Biol. Chem. 258: 9593-9596, 1983. PubMed: 6688416

Melton DW, et al. Structure, expression, and mutation of the hypoxanthine phosphoribosyltransferase gene. Proc. Natl. Acad. Sci. USA 81: 2147-2151, 1984. PubMed: 6326107

C. Thomas Caskey, personal communication

梅經理 17280875617 1438578920
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周經理 17757487661 1296385441
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沈經理 19548299266 2662369050
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