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contains sequence kinectin

Construct size (kb): 7.300000190734863

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-7Zf(+)
Intact vector size: 3.000
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: ApaI AatII SphI XbaI XhoI EcoRI KpnI SmaI AsuII ClaII
HindIII BamHI SacI BstXI NsiI
Polylinker sites: ApaI AatII SphI XbaI XhoI EcoRI KpnI SmaI AsuII ClaII
HindIII BamHI SacI BstXI NsiI
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, SP6, T7
replicon: pMB1, f1
terminator: none
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: KIN*
Gene name: kinectin
Contains complete coding sequence?: Y
Type of DNA: cDNA
Insert end: Modification: EcoRI adaptor
Insert end: Modification: EcoRI adaptor
Insert size (kb): 4.309
Cross references: DNA Seq. Acc.: Z22551
Nucleotides 1-4309 of the insert correspond to
nucleotides 1-4309 of Z22551.

Insert lengths(kb): 4.309000015258789

Gene product: kinectin [KIN*]

Target Gene: kinectin

Restriction digests of the clone give the following sizes (kb): BamHI--7.0, 1.0; EcoRI--4.7, 3.4; XhoI--4.4, 3.7.

Insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.85; BglII--3.76; HindIII--0.20, 0.70, 1.67; KpnI--0.62; NcoI--0.07; PstI--1.40, 3.21; PvuII--0.54, 2.95; XbaI--3.42; XhoI--0.31.

Recommendation for verification: EcoRI--4.3, 3.0; BamHI--6.4, 0.9; HindIII--5.6, 1.0, 0.5, 0.2; XbaI--6.4, 0.9; XhoI--4.0, 3.3.