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Size (kb): 43.1

Vector: Charon BS(-) (phage, lambda - replacement)

Promoters: Promoter for in vitro transcription T3

Construction: Charon 15, pBluescript KS M13(-)

Marker(s):ampR

Construct size (kb): 43.1

Features: insert detection: lacZ'
marker(s): ampR
other: left arm
other: pBluescript KS -
other: right arm
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: f1
replicon: pMB1
MCS: NotI...KpnI
MCS: SacI...NotI

expression vector

lambda vector allowing conversion to plasmid

vector containing primer sites useful for sequencing

vector for constructing cDNA libraries

vector permitting production of single-stranded DNA

vector permitting visual detection of recombinants

Restriction digests of the clone give the following sizes (kb): BamHI--31.0, 15.5, 6.0; EcoRI--25.0 (doublet); SalI--27.0, 13.5, 9.4; XhoI--27.0, 13.0, 9.2.

Vector for high-efficiency directional cloning. Clones can be converted to plasmids by recircularization after a NotI digest.

Charon BS(-) (ATCC 77446) and Charon BS(+) (ATCC 77447) differ in the orientation of the M13 origin of replication.