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NCI-H1568 [H1568]
NCI-H1568 [H1568]
規(guī)格:
貨期:
編號:B161439
品牌:Mingzhoubio

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產(chǎn)品名稱 NCI-H1568 [H1568]
商品貨號 B161439
Organism Homo sapiens, human
Tissue lung; derived from metastatic site: lymph node
Product Format frozen
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease adenocarcinoma; non-small cell lung cancer
Age 48 years
Gender female
Ethnicity Caucasian
Images
Derivation The line was established in December 1986 from lymph node metastasis of lung.
Clinical Data 48 years
Caucasian
female
The patient was a smoker.
60 pack years.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 10,12
D5S818: 9
D7S820: 8,9
THO1: 9
TPOX: 8,11
vWA: 16,17
Name of Depositor AF Gazdar, JD Minna
Deposited As Homo sapiens
Year of Origin 1986
References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

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于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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