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Origami 2 (DE3) pLysS Strain
Origami 2 (DE3) pLysS Strain
規(guī)格:
貨期:
編號(hào):B98211
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Origami 2 (DE3) pLysS Strain
商品貨號(hào) B98211
規(guī)格 100μl
儲(chǔ)存 -20℃
基因型 Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139 ahpC galE galK rpsL F′[lac+ lacIq pro] (DE3) gor522::Tn10 trxB pLysS (CamR, StrR, TetR)
基本信息 抗性:Str/Chl/tet 培養(yǎng)基:LB 菌株類別:大腸桿菌 培養(yǎng)條件:37℃,有氧,LB 質(zhì)粒轉(zhuǎn)化:42℃熱激 保存方式:20%甘油,-20℃ 基本應(yīng)用:用于蛋白表達(dá) 備注:
菌株簡(jiǎn)介 Origami 2 host strains are K-12 derivatives that have mutations in both the thioredoxin reductase (trxB) and glutathione reductase (gor) genes, which greatly enhance disulfide bond formation in the E. coli cytoplasm. The Origami 2 strains are kanamycin sensitive; like the original Origami strains, the gor mutation is still selected for by tetracycline. To reduce the possibility of disulfide bond formation between molecules, strains containing mutations in trxB and gor are recommended only for the expression of proteins that require disulfide bond formation for proper folding. DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG. pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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